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ACTN2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - ACTN2 Antibody (Center) AP20894a
    Western blot analysis of lysates from A431, Hela, HepG2, NCI-H1299, mouse NIH/3T3 cell line, human skeletal muscle tissue lysate (from left to right), using ACTN2 Antibody (Center) (Cat. #AP20894a). AP20894a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P35609, Q9JI91
Other Accession P20111, Q3ZC55, P35609, Q9JI91
Reactivity Human, Mouse
Predicted Bovine, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Other Names Alpha-actinin-2, Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein, ACTN2
Target/Specificity This ACTN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 352-386 amino acids from the Central region of human ACTN2.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsACTN2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations (0)
citation

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Background

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

References

Beggs A.H.,et al.J. Biol. Chem. 267:9281-9288(1992).
Tiso N.,et al.Biochem. Biophys. Res. Commun. 265:256-259(1999).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Faulkner G.,et al.J. Cell Biol. 146:465-475(1999).

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$ 385.00
$ 150.00
Cat# AP20894a
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